Crispr , dubbed as the discovery of the century has been used in variety of applications. It is used in genome editing, epigenetic modification, modulation of gene expression, locus identification (CASFISH). Recently it has also been used in drug delivery. Crispr has revolutionised biology. Traditional fluorescent in situ hybridization (FISH) requires DNA denaturation, while fluorescent labeled- dCas9 assembled in vitro with sgRNA (CASFISH) detects genomic loci only in fixed samples. Here, this study report a live imaging approach, CRISPR LiveFISH for the first time, that allows real-time study of various chromosomal functions in living cells. They developed CRISPR LiveFISH, an approach that deploys fluorescent gRNA probes assembled with dCas9 to target and label genomic sequences in living cells. They tested CRISPR LiveFISH on cells derived from patients with Patau Syndrome, a Chr13 trisomy genetic disorder that results in organ defects, intellectual and physical ...
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